Purified proteins stand as a central focus in numerous research laboratories, where they are used for a wide spectrum of applications ranging from 3D structural analysis, through X-ray, cryoEM, and NMR studies, to in-depth investigations into interactions (protein-protein, protein-ligand, or protein-nucleic acid) and cell biology experiments. Furthermore, these proteins play a crucial role in characterizing biological (e.g. enzymatic) activities. Beyond the realm of academic research, purified proteins serve as efficient tools in the therapeutic and diagnostic landscape, finding applications in various pharmaceutical industries. To ensure the reliability of experimental data in these studies, stringent protein quality controls are imperative. In 2021, the collaborative efforts of the ARBRE-MOBIEU and P4EU networks resulted in the publication of comprehensive guidelines for evaluating purified proteins in life sciences and medical trials (de Marco et al., 2021; Berrow et al., 2021). The objective of this course is to familiarize participants with these guidelines for protein quality control and to provide an overview of the technologies essential for enhancing the accuracy and reproducibility of data obtained with purified proteins. Throughout the course, participants will have access to the theoretical foundations of these technologies and will also gain hands-on experience with them, ensuring a comprehensive learning experience.

This course is designed for scientists currently using or intending to use purified proteins in their laboratory experiments. By the end of the course, participants should be familiar with a variety of techniques applicable to purified proteins, ultimately enhancing the reliability and reproducibility of data in downstream applications involving these proteins.

We offer a 4-day course comprising lectures on protein expression and purification techniques, and methods for assessing the quality of purified protein samples. Additionally, participants will have hands-on practical sessions demonstrating how to apply these experiments for protein quality control.

Topics included in the course:

Application

This course is limited to 15 participants. Applicants will be selected on the basis of a motivation letter  (max. 1 page) and their CV. Please also provide a letter of support from your supervisor. Note that priority will be given to junior researchers, namely PhD students and postdocs. 

Deadline for registration is March 15^th, 2024

Application should be sent to amatagne@uliege.be

Registration

Registration fees (500€) include admission, course materials, lunches and coffee breaks. Payement should be after acceptance to the course, which will be announced approximatly 2-3 weeks after the application deadline. Participants must book and pay for their own accommodation and travel expenses.